Part:BBa_J07009

toxicity-gene activator from Vibrio cholerae

shortened BBa_J07008. Typical ToxR-periplasm-region subsitutions are done at aa 211. explanation of ToxR domains at http://model.mit.edu/igem/images/d/d7/Kolmar95.pdf

ToxR gene with shortened periplasmic terminus. The downstream portion of this gene was specified to be fusable to another protein.

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Usage and Biology

This transcription activator controls cholera toxin, pilus colonization factor and outer membrane protein expression in V.cholerae. It binds to the 5'-TTTTGAT-3' tandemly repeated DNA sequence in the cholera toxin promoter region. ToxS interacts with the C-terminal periplasmic domain of toxR, stimulating its activity. It activates transcription at the promoters for tcpI and tcpA and this is presumably via toxT.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]